diff --git a/ecs/jskult-webapp/.env.example b/ecs/jskult-webapp/.env.example
index 10844a21..6afc42c6 100644
--- a/ecs/jskult-webapp/.env.example
+++ b/ecs/jskult-webapp/.env.example
@@ -23,6 +23,7 @@ AWS_REGION=ap-northeast-1
 AUTHORIZE_ENDPOINT=oauth2/authorize
 TOKEN_ENDPOINT=oauth2/token
 BIO_SEARCH_RESULT_MAX_COUNT=35000
+BIO_EXCEL_RESULT_MAX_COUNT=15000
 ULTMARC_SEARCH_RESULT_MAX_COUNT=500
 SESSION_EXPIRE_MINUTE=20
 LOG_LEVEL=DEBUG
\ No newline at end of file
diff --git a/ecs/jskult-webapp/Dockerfile b/ecs/jskult-webapp/Dockerfile
index d7590655..aea8870f 100644
--- a/ecs/jskult-webapp/Dockerfile
+++ b/ecs/jskult-webapp/Dockerfile
@@ -16,4 +16,4 @@ RUN \
 
 COPY src ./src
 
-CMD ["gunicorn", "src.main:app", "-w", "4", "-k" ,"uvicorn.workers.UvicornWorker", "-b", "0.0.0.0:80", "--timeout", "300"]
+CMD ["gunicorn", "src.main:app", "-w", "1", "-k" ,"uvicorn.workers.UvicornWorker", "-b", "0.0.0.0:80", "--timeout", "300"]
diff --git a/ecs/jskult-webapp/src/controller/bio_download.py b/ecs/jskult-webapp/src/controller/bio_download.py
index 454ff857..f076f28b 100644
--- a/ecs/jskult-webapp/src/controller/bio_download.py
+++ b/ecs/jskult-webapp/src/controller/bio_download.py
@@ -51,7 +51,7 @@ async def download_bio_data(
         return {'status': 'batch_processing'}
     # 生物由来データを検索
     # 検索に使用したクエリも取得
-    search_result_df, query = _search_bio_data(bio_service, search_param, download_param.user_id)
+    search_result_df, query = _search_bio_data(bio_service, search_param, download_param)
     # アクセスログを記録
     bio_service.write_access_log(query, search_param, download_param.user_id, current_timestamp, download_file_name)
 
@@ -61,7 +61,9 @@ async def download_bio_data(
         return {'status': 'ok', 'download_url': None}
 
     # ファイルを書き出し(Excel or CSV)
-    local_file_path = _write_bio_data_to_file(bio_service, download_param, search_result_df, download_file_name)
+    local_file_path = await _write_bio_data_to_file(bio_service, download_param, search_result_df, download_file_name)
+
+    logger.info('ファイル書き出し完了')
 
     # ローカルファイルからS3にアップロードし、ダウンロード用URLを取得する
     try:
@@ -99,10 +101,19 @@ async def download_bio_data(
     return json_response
 
 
-def _search_bio_data(bio_service: BioViewService, search_param: BioModel, user_id: str) -> pd.DataFrame:
+def _search_bio_data(
+    bio_service: BioViewService,
+    search_param: BioModel,
+    download_param: BioDownloadModel
+) -> pd.DataFrame:
     try:
         # 生物由来データを検索
-        search_result_df, query = bio_service.search_download_bio_data(search_param)
+        # Excelの場合、出力件数を絞る
+        if download_param.ext == 'xlsx':
+            search_result_df, query = bio_service.search_download_bio_data(
+                search_param, limitation=environment.BIO_EXCEL_RESULT_MAX_COUNT)
+        elif download_param.ext == 'csv':
+            search_result_df, query = bio_service.search_download_bio_data(search_param)
     except DBException as e:
         raise HTTPException(
             status_code=status.HTTP_500_INTERNAL_SERVER_ERROR,
@@ -112,7 +123,7 @@ def _search_bio_data(bio_service: BioViewService, search_param: BioModel, user_i
     return search_result_df, query
 
 
-def _write_bio_data_to_file(
+async def _write_bio_data_to_file(
     bio_service: BioViewService,
     download_param: BioDownloadModel,
     df: pd.DataFrame,
@@ -122,11 +133,11 @@ def _write_bio_data_to_file(
     local_file_path = ''
     if download_param.ext == 'xlsx':
         logger.info('今回はExcelファイルに出力する')
-        local_file_path = bio_service.write_excel_file(
+        local_file_path = await bio_service.write_excel_file(
             df, download_param.user_id, download_file_name=download_file_name)
     elif download_param.ext == 'csv':
         logger.info('今回はCSVファイルに出力する')
-        local_file_path = bio_service.write_csv_file(
+        local_file_path = await bio_service.write_csv_file(
             df, download_param.user_id, header=constants.BIO_CSV_HEADER, download_file_name=download_file_name)
 
     return local_file_path
diff --git a/ecs/jskult-webapp/src/controller/master_mainte.py b/ecs/jskult-webapp/src/controller/master_mainte.py
index 7543c12e..ac9fa50b 100644
--- a/ecs/jskult-webapp/src/controller/master_mainte.py
+++ b/ecs/jskult-webapp/src/controller/master_mainte.py
@@ -234,7 +234,7 @@ def inst_emp_csv_download_view(
 
 
 @router.post('/download', response_class=HTMLResponse)
-async def inst_emp_csv_download(
+def inst_emp_csv_download(
     request: Request,
     csv_download_form: Optional[MasterMainteCsvDlModel] = Depends(MasterMainteCsvDlModel.as_form),
     master_mainte_service: MasterMainteService = Depends(get_service(MasterMainteService)),
diff --git a/ecs/jskult-webapp/src/depends/database.py b/ecs/jskult-webapp/src/depends/database.py
index 65a8a967..2dea5dab 100644
--- a/ecs/jskult-webapp/src/depends/database.py
+++ b/ecs/jskult-webapp/src/depends/database.py
@@ -5,4 +5,9 @@ from src.db.database import Database
 
 def get_database(request: Request) -> Database:
     # medaca_routerでDB接続エンジンが初期化される
-    return request.app.state._db
+    db = getattr(request.app.state, '_db', None)
+    # uvicornのワーカーが起動したタイミングでは、dbがセットされていないので、ここでセットここでセットする
+    if db is None:
+        db = Database.get_instance()
+        setattr(request.app.state, '_db', db)
+    return db
diff --git a/ecs/jskult-webapp/src/model/view/bio_view_model.py b/ecs/jskult-webapp/src/model/view/bio_view_model.py
index bd43cd7a..7ad9e8cb 100644
--- a/ecs/jskult-webapp/src/model/view/bio_view_model.py
+++ b/ecs/jskult-webapp/src/model/view/bio_view_model.py
@@ -19,6 +19,7 @@ class BioViewModel(BaseModel):
     phm_models: list[PharmacyProductMasterModel]
     bio_data: Optional[list[BisDisplayModel]] = None
     form_data: BioModel = None
+    excel_max_lines: int = str(environment.BIO_EXCEL_RESULT_MAX_COUNT)
 
     def display_wholesaler_names(self):
         display_names = [
diff --git a/ecs/jskult-webapp/src/repositories/bio_sales_lot_repository.py b/ecs/jskult-webapp/src/repositories/bio_sales_lot_repository.py
index 00dc4b68..30e2b562 100644
--- a/ecs/jskult-webapp/src/repositories/bio_sales_lot_repository.py
+++ b/ecs/jskult-webapp/src/repositories/bio_sales_lot_repository.py
@@ -81,12 +81,12 @@ class BioSalesLotRepository(BaseRepository):
         finally:
             self._database.disconnect()
 
-    def fetch_as_data_frame(self, parameter: BioModel):
+    def fetch_as_data_frame(self, parameter: BioModel, limitation: int):
         try:
             self._database.connect()
             logger.debug('DB参照実行')
             where_clause = self.__build_condition(parameter)
-            query = self.FETCH_SQL.format(where_clause=where_clause, limit=environment.BIO_SEARCH_RESULT_MAX_COUNT + 1)
+            query = self.FETCH_SQL.format(where_clause=where_clause, limit=limitation)
             logger.debug(f'SQL: {query}')
             df = self._to_data_frame(query, parameter)
             logger.debug(f'count= {len(df.index)}')
diff --git a/ecs/jskult-webapp/src/services/bio_view_service.py b/ecs/jskult-webapp/src/services/bio_view_service.py
index dd348d06..cf9961ac 100644
--- a/ecs/jskult-webapp/src/services/bio_view_service.py
+++ b/ecs/jskult-webapp/src/services/bio_view_service.py
@@ -1,3 +1,5 @@
+import asyncio
+import functools
 import os
 import shutil
 from datetime import datetime
@@ -69,9 +71,15 @@ class BioViewService(BaseService):
 
         return display_bio_data
 
-    def search_download_bio_data(self, search_params: BioModel):
+    def search_download_bio_data(
+            self,
+            search_params: BioModel,
+            limitation=(environment.BIO_SEARCH_RESULT_MAX_COUNT + 1)
+    ):
         # 生物由来データをダウンロードするために、DBから検索した結果をデータフレームに変換
-        bio_sales_data_frame, query = self.bio_sales_repository.fetch_as_data_frame(parameter=search_params)
+        bio_sales_data_frame, query = self.bio_sales_repository.fetch_as_data_frame(
+            parameter=search_params, limitation=limitation
+        )
         return bio_sales_data_frame, query
 
     def write_access_log(
@@ -125,7 +133,9 @@ class BioViewService(BaseService):
         # S3にアップロード
         self.upload_bio_access_log_file(access_log_file_path)
 
-    def write_excel_file(self, data_frame: pd.DataFrame, user_id: str, download_file_name: str):
+    async def write_excel_file(self, data_frame: pd.DataFrame, user_id: str, download_file_name: str):
+        # 非同期処理用のイベントループを取得
+        loop = asyncio.get_event_loop()
         # Excelに書き込み
         output_file_path = os.path.join(constants.BIO_TEMPORARY_FILE_DIR_PATH, download_file_name)
 
@@ -141,11 +151,27 @@ class BioViewService(BaseService):
             # `sheet_name`引数を省略した場合は、「Sheet1」に書き込む。
             # DF内のヘッダと連番を書き込みたくない場合、`header`と`index`をFalseに指定する。
             # `startrow`と`startcol`で、Excelの書き込み位置を決定する。省略した場合はA1セルから書く。
-            data_frame.to_excel(writer, header=False, index=False, startrow=1, startcol=0)
+
+            data_frame_chunk_size = 2000  # 各部分データフレームのサイズ
+            is_first = True  # ループの初回
+            data_frame_range = len(data_frame)
+            # 指定行数ごとにデータを分割して処理
+            for i in range(0, data_frame_range, data_frame_chunk_size):
+                chunk_df = data_frame.iloc[i:i + data_frame_chunk_size]
+                start_row = i + 1
+                # 1回目の分割では、ヘッダ部分を上書きしないようにする
+                if is_first:
+                    is_first = False
+                    start_row = 1
+                await loop.run_in_executor(None, functools.partial(
+                    chunk_df.to_excel, writer,
+                    header=False, index=False, startrow=start_row, startcol=0))
 
         return output_file_path
 
-    def write_csv_file(self, data_frame: pd.DataFrame, user_id: str, header: list[str], download_file_name: str):
+    async def write_csv_file(self, data_frame: pd.DataFrame, user_id: str, header: list[str], download_file_name: str):
+        # 非同期処理用のイベントループを取得
+        loop = asyncio.get_event_loop()
         # csvに書き込み
         output_file_path = os.path.join(constants.BIO_TEMPORARY_FILE_DIR_PATH, download_file_name)
         # 横長のDataFrameとするため、ヘッダーの加工処理
@@ -156,7 +182,9 @@ class BioViewService(BaseService):
         header_df = pd.DataFrame([header_data], index=None)
         output_df = pd.concat([header_df, data_frame])
         # ヘッダー行としてではなく、1レコードとして出力する
-        output_df.to_csv(output_file_path, index=False, header=False, encoding="utf-8_sig")
+        await loop.run_in_executor(None, functools.partial(
+            output_df.to_csv, output_file_path,
+            index=False, header=False, encoding="utf-8_sig"))
 
         return output_file_path
 
diff --git a/ecs/jskult-webapp/src/system_var/environment.py b/ecs/jskult-webapp/src/system_var/environment.py
index ec24aeb9..a03b02d5 100644
--- a/ecs/jskult-webapp/src/system_var/environment.py
+++ b/ecs/jskult-webapp/src/system_var/environment.py
@@ -20,6 +20,7 @@ DB_PASSWORD = os.environ['DB_PASSWORD']
 DB_SCHEMA = os.environ['DB_SCHEMA']
 
 BIO_SEARCH_RESULT_MAX_COUNT = int(os.environ.get('BIO_SEARCH_RESULT_MAX_COUNT', 35000))
+BIO_EXCEL_RESULT_MAX_COUNT = int(os.environ.get('BIO_EXCEL_RESULT_MAX_COUNT', 15000))
 ULTMARC_SEARCH_RESULT_MAX_COUNT = int(os.environ.get('ULTMARC_SEARCH_RESULT_MAX_COUNT', 500))
 SESSION_EXPIRE_MINUTE = int(os.environ['SESSION_EXPIRE_MINUTE'])
 
diff --git a/ecs/jskult-webapp/src/templates/_modal.html b/ecs/jskult-webapp/src/templates/_modal.html
index 6477db10..31d7df97 100644
--- a/ecs/jskult-webapp/src/templates/_modal.html
+++ b/ecs/jskult-webapp/src/templates/_modal.html
@@ -1,4 +1,4 @@
-{% with 
+{% with
     icon_data = {
         'info': {
             'alert': 'alert-primary',
@@ -18,10 +18,14 @@
           <button type="button" id="{{modal_id}}_close" onclick="{{modal_close_event}}" class="btn-close" data-bs-dismiss="modal" aria-label="Close"></button>
         </div>
         <div class="modal-body">
-          <img src="{{icon_data[icon_key]['icon_src']}}" loading="lazy" style="width: 50px;">
-          {% autoescape False %}
-          {{message}}
-          {% endautoescape %}
+          <div style="display: inline-block;">
+            <img src="{{icon_data[icon_key]['icon_src']}}" loading="lazy" style="width: 50px;">
+          </div>
+          <div style="display: inline-block;">
+            {% autoescape False %}
+            {{message}}
+            {% endautoescape %}
+          </div>
         </div>
         <div class="modal-footer">
           {% for button in buttons%}
diff --git a/ecs/jskult-webapp/src/templates/bioSearchList.html b/ecs/jskult-webapp/src/templates/bioSearchList.html
index 7f9fa247..a7035b28 100644
--- a/ecs/jskult-webapp/src/templates/bioSearchList.html
+++ b/ecs/jskult-webapp/src/templates/bioSearchList.html
@@ -381,7 +381,7 @@
     {% with
         modal_id='modal_xlsx',
         modal_title='確認',
-        message='生物由来卸販売データ一覧をExcel出力しますか？',
+        message='生物由来卸販売データ一覧をExcel出力しますか？<br>※出力件数が' + bio.excel_max_lines + '件に制限されます。全件出力したい場合はCSVで出力してください。',
         icon_key='info',
         modal_close_event='',
         buttons = [
diff --git a/s3/config/jskult/task_settings/web_task_settings.env b/s3/config/jskult/task_settings/web_task_settings.env
index 9f6b542a..6746ac01 100644
--- a/s3/config/jskult/task_settings/web_task_settings.env
+++ b/s3/config/jskult/task_settings/web_task_settings.env
@@ -3,5 +3,6 @@ AWS_REGION=ap-northeast-1
 AUTHORIZE_ENDPOINT=oauth2/authorize
 TOKEN_ENDPOINT=oauth2/token
 BIO_SEARCH_RESULT_MAX_COUNT=35000
+BIO_EXCEL_RESULT_MAX_COUNT=15000
 SESSION_EXPIRE_MINUTE=20
 ULTMARC_SEARCH_RESULT_MAX_COUNT=500